14-1957-37
antibody from Invitrogen Antibodies
Targeting: CCR5
CC-CKR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-1957-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions. Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunocytochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates. Applications Tested: This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioT21/8 (T21/8)
- Vial size
- 2 mg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Pericytes augment glioblastoma cell resistance to temozolomide through CCL5-CCR5 paracrine signaling.
CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells.
Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Natural killer cells generated from cord blood hematopoietic progenitor cells efficiently target bone marrow-residing human leukemia cells in NOD/SCID/IL2Rg(null) mice.
Zhang XN, Yang KD, Chen C, He ZC, Wang QH, Feng H, Lv SQ, Wang Y, Mao M, Liu Q, Tan YY, Wang WY, Li TR, Che LR, Qin ZY, Wu LX, Luo M, Luo CH, Liu YQ, Yin W, Wang C, Guo HT, Li QR, Wang B, Chen W, Wang S, Shi Y, Bian XW, Ping YF
Cell research 2021 Oct;31(10):1072-1087
Cell research 2021 Oct;31(10):1072-1087
CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells.
Fox JM, Kasprowicz R, Hartley O, Signoret N
Journal of leukocyte biology 2015 Jul;98(1):59-71
Journal of leukocyte biology 2015 Jul;98(1):59-71
Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.
Ray-Saha S, Huber T, Sakmar TP
Biochemistry 2014 Mar 4;53(8):1302-10
Biochemistry 2014 Mar 4;53(8):1302-10
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Hartmann N, Giese NA, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Natural killer cells generated from cord blood hematopoietic progenitor cells efficiently target bone marrow-residing human leukemia cells in NOD/SCID/IL2Rg(null) mice.
Cany J, van der Waart AB, Tordoir M, Franssen GM, Hangalapura BN, de Vries J, Boerman O, Schaap N, van der Voort R, Spanholtz J, Dolstra H
PloS one 2013;8(6):e64384
PloS one 2013;8(6):e64384
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD195 (CCR5) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.