Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-53244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCAR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SPLAEHRPDA YQDVSIHGTL PRKKKGPPPI RSCDDFSHMG TLPHSKSPRQ NSPVTQDGIQ ESPWQDRHGE TFTFRDPHLL DPTVEYVKFS KERHIMDRTP EKLKKEL Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCAR3 in human cell lines PC-3 and HEK293 using a BCAR3 Polyclonal Antibody (Product # PA5-53244). Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of BCAR3 in human cell line U-251 MG shows positivity in plasma membrane, cytoplasm & centrosome. Samples were probed using a BCAR3 Polyclonal Antibody (Product # PA5-53244).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCAR3 in human kidney tissue shows moderate cytoplasmic positivity in tubular cells. Samples were probed using a BCAR3 Polyclonal Antibody (Product # PA5-53244).