HPA014858

antibody from Atlas Antibodies

Targeting: BCAR3 AND-34, NSP2, SH2D3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA014858

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014858, RRID:AB_1845302

Product name

Anti-BCAR3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SPLAEHRPDAYQDVSIHGTLPRKKKGPPPIRSCDD
FSHMGTLPHSKSPRQNSPVTQDGIQESPWQDRHGE
TFTFRDPHLLDPTVEYVKFSKERHIMDRTPEKLKK
EL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines PC-3 and HEK293 using Anti-BCAR3 antibody. Corresponding BCAR3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line PC-3.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & centrosome.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.

Sample type

HUMAN