Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-53434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bisphosphoglycerate mutase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RRSYNVTPPP IEESHPYYQE IYNDRRYKVC DVPLDQLPRS ESLKDVLERL LPYWNERIAP EVLRGKTILI SAHGNSSRAL LKHLEGISDE DIINITLPTG VPILLELDEN LRAVGPHQFL GDQEAIQAAI KKVEDQGKVK QA Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 90%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bisphosphoglycerate mutase in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bisphosphoglycerate mutase in human cell line U-251 MG using a Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Bisphosphoglycerate mutase in mouse cell line NIH-3T3 and rat cell line NBT-II using a Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Bisphosphoglycerate mutase in human cell line U-2 OS shows positivity in nucleus & nucleoli. Samples were probed using a Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bisphosphoglycerate mutase in human placenta and pancreas tissues using Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434). Corresponding BPGM RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bisphosphoglycerate mutase in human placenta using Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Bisphosphoglycerate mutase in human pancreas using Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-53434) shows low expression as expected.