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LearnMA5-49186
antibody from Invitrogen Antibodies
Targeting: SLC4A1
AE1, CD233, DI, EPB3, FR, RTA1A, SW, WD, WR
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-49186 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Band 3 Monoclonal Antibody (5G2G7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL. Human SLC4A1 shares 75.7% and 74.5% amino acid (aa) sequence identity with and rat SLC4A1, respectively. Positive Control - WB: human K562 whole cell. IHC: human spleen tissue. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G2G7
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Band 3 in paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with Band 3 Monoclonal antibody (Product # MA5-49186) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Band 3 in HepG2 cells (Blue line). The cells were blocked with 10% normal goat serum, and then incubated with SLC4A1 monoclonal antibody (Product # MA5-49186) (1 µg/1x10^6 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10^6 cells) used under the same conditions. Unlabeled sample (Red line) was also used as a control. DyLight®488 conjugated goat anti-mouse IgG (5-10 μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C.
