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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Immunohistochemistry [1]
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- Product number
- PB9737 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PPP1R12A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat;Monkey. PPP1R12A information: Molecular Weight: 115281 MW; Subcellular Localization: Cytoplasm . Along actomyosin filaments and stress fibers; Tissue Specificity: Expressed in striated muscles, specifically in type 2a fibers (at protein level).
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells.
Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling.
The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia.
Dong Q, Luo Y, Yin Y, Ma Y, Yu Y, Wang L, Yang H, Pan Y, Zhang D
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
Glucagon-like peptide 1 reverses myocardial hypertrophy through cAMP/PKA/RhoA/ROCK2 signaling.
Fan S, Xiong Q, Zhang X, Zhang L, Shi Y
Acta biochimica et biophysica Sinica 2020 Jun 20;52(6):612-619
Acta biochimica et biophysica Sinica 2020 Jun 20;52(6):612-619
The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia.
Yan YY, Wang XM, Jiang Y, Chen H, He JT, Mang J, Shao YK, Xu ZX
Neural regeneration research 2015 Sep;10(9):1441-9
Neural regeneration research 2015 Sep;10(9):1441-9
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). PPP1R12A was detected in paraffin-embedded section of Human Glioma Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
