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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- R30766 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Myosin Phosphatase Antibody / MYPT1 / PPP1R12A
- Antibody type
- Polyclonal
- Description
- This highly specific Myosin Phosphatase antibody is suitable for use in Western blot/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Myosin Phosphatase antibody and Lane 1: rat liver; 2: human 293T; 3: (h) HeLa cell lysate. Observed size: 110~130KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) COS-7 (monkey), 5) Raji, 6) K562, 7) Caco-2 and 8) HepG2 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat C6, 3) mouse liver and 4) mouse NIH3T3 lysate with Myosin Phosphatase antibody. Expected molecular weight: 110~130 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human SiHa cells with Myosin Phosphatase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-251 MG cells with Myosin Phosphatase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with Myosin Phosphatase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Myosin Phosphatase antibody.
