PA5-87459

antibody from Invitrogen Antibodies

Targeting: PPP1R12A M130, MBS, MYPT1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87459

Provider

Invitrogen Antibodies

Product name

MYPT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERIMLRD ARQWLNSGHI NDVRHAKSGG; Positive Samples: U-87MG, LO2, Jurkat, HeLa; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.27 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MYPT1 in HeLa cells. Samples were incubated with MYPT1 Polyclonal antibody (Product # PA5-87459) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MYPT1 in NIH/3T3 cells. Samples were incubated with MYPT1 Polyclonal antibody (Product # PA5-87459) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MYPT1 in PC-12 cells. Samples were incubated with MYPT1 Polyclonal antibody (Product # PA5-87459) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MYPT1 in U2OS cells. Samples were incubated with MYPT1 Polyclonal antibody (Product # PA5-87459) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MYPT1 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg MYPT1 Polyclonal antibody (Product # PA5-87459). Western blot was performed from the immunoprecipitate using MYPT1 Polyclonal antibody (Product # PA5-87459) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of MYPT1 was performed in 200 µg extracts of HeLa cells using MYPT1 Polyclonal Antibody (Product # PA5-87459). Western blot was performed from the immunoprecipitate using MYPT1 Polyclonal Antibody.