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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [3]
- Other assay [6]
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- Product number
- PA5-17164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYPT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other family members.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 5 µg/mL
- Storage
- -20°C
Submitted references The IGF1 Receptor Is Involved in Follicle-Stimulating Hormone Signaling in Porcine Neonatal Sertoli Cells.
Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1.
Increased degradation of MYPT1 contributes to the development of tolerance to nitric oxide in porcine pulmonary artery.
Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance.
Cannarella R, Arato I, Condorelli RA, Luca G, Barbagallo F, Alamo A, Bellucci C, Lilli C, La Vignera S, Calafiore R, Mancuso F, Calogero AE
Journal of clinical medicine 2019 Apr 27;8(5)
Journal of clinical medicine 2019 Apr 27;8(5)
Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1.
Ying L, Xu X, Liu J, Dou D, Yu X, Ye L, He Q, Gao Y
Pflugers Archiv : European journal of physiology 2012 Feb;463(2):257-68
Pflugers Archiv : European journal of physiology 2012 Feb;463(2):257-68
Increased degradation of MYPT1 contributes to the development of tolerance to nitric oxide in porcine pulmonary artery.
Ma H, He Q, Dou D, Zheng X, Ying L, Wu Y, Raj JU, Gao Y
American journal of physiology. Lung cellular and molecular physiology 2010 Jul;299(1):L117-23
American journal of physiology. Lung cellular and molecular physiology 2010 Jul;299(1):L117-23
Degradation of leucine zipper-positive isoform of MYPT1 may contribute to development of nitrate tolerance.
Dou D, Ma H, Zheng X, Ying L, Guo Y, Yu X, Gao Y
Cardiovascular research 2010 Apr 1;86(1):151-9
Cardiovascular research 2010 Apr 1;86(1):151-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MYPT1 in extracts from C6 and COS cells using MYPT1 polyclonal antibody (Product # PA5-17164).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MYPT1 was achieved by transfecting HeLa cells with MYPT1 specific siRNAs (Silencer® select Product # s9237, s9235). Western blot analysis (Fig. a) was performed using whole cell extracts from the MYPT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-MYPT1 Polyclonal Antibody (Product # PA5-17164, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MYPT1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-2 OS (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), HEK293 (Lane 4) and U-87 MG (Lane 5). The blot was probed with Anti-MYPT1 Polyclonal Antibody (Product # PA5-17164, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 130kDa band corresponding to MYPT1 was seen in all cell lines tested. Additional uncharacterized bands were also observed in the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Insulin-like growth factor 1 receptor (IGF1R) is required for the Follicle-stimulating hormone (FSH)-induced myosin-phosphatase 1 (MYPT1) phosphorylation. ( a ) Immunoblots and ( b ) densitometric analysis of phosphorilated myosin-phosphatase 1 (pMYPT1), MYPT1 and Glyceraldehyde-3-Phosphate Dehydrogenase (GADPH) from Sertoli cells alone (control), or incubated with hpFSH alone or pre-treated with the IGF1R inhibitor NVP-AEW541 and/or protein phosphatase 1ss (PP1ss) inhibitor tautomycin and then incubated with hpFSH. Data represent the mean +- standard error of the mean (SEM) (* p < 0.05 vs. controls and + p < 0.05 vs. FSH treatment alone) (one-way ANOVA) of three independent experiments, each performed in triplicate.
