Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- PA1681 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Myosin Phosphatase/PPP1R12A Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Protein phosphatase 1 regulatory subunit 12A(PPP1R12A) detection. Tested with WB, ICC/IF, FCM in Human;Mouse;Rat;Monkey.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references RhoA/ROCK1 regulates the mitochondrial dysfunction through Drp1 induced by Porphyromonas gingivalis in endothelial cells.
Dong Q, Luo Y, Yin Y, Ma Y, Yu Y, Wang L, Yang H, Pan Y, Zhang D
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
Journal of cellular and molecular medicine 2023 Aug;27(15):2123-2135
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Hela cells using anti-PPP1R12A antibody (PA1681 ).Overlay histogram showing Hela cells stained with PA1681 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PA1681 ,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image