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LearnPA5-34934
antibody from Invitrogen Antibodies
Targeting: SETD2
FLJ23184, HIF-1, HYPB, KIAA1732, KMT3A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-34934 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SETD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, K562.
- Concentration
- 0.96 mg/mL
Submitted references The Role of circRNA-SETD2/miR-519a/PTEN Axis in Fetal Birth Weight through Regulating Trophoblast Proliferation.
Snail heterogeneity in clear cell renal cell carcinoma.
NF-κB-dependent and -independent epigenetic modulation using the novel anti-cancer agent DMAPT.
Wang D, Na Q, Song G, Wang Y, Wang Y
BioMed research international 2020;2020:9809632
BioMed research international 2020;2020:9809632
Snail heterogeneity in clear cell renal cell carcinoma.
Zaldumbide L, Erramuzpe A, Guarch R, Pulido R, Cortés JM, López JI
BMC cancer 2016 Mar 8;16:194
BMC cancer 2016 Mar 8;16:194
NF-κB-dependent and -independent epigenetic modulation using the novel anti-cancer agent DMAPT.
Nakshatri H, Appaiah HN, Anjanappa M, Gilley D, Tanaka H, Badve S, Crooks PA, Mathews W, Sweeney C, Bhat-Nakshatri P
Cell death & disease 2015 Jan 22;6(1):e1608
Cell death & disease 2015 Jan 22;6(1):e1608
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SETD2 using 30 µg of A) Raji and B) K562 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SETD2 polyclonal antibody (Product # PA5-34934) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SETD2 Polyclonal Antibody (Product # PA5-34934). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETD2 Polyclonal Antibody (Product # PA5-34934) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETD2 Polyclonal Antibody detects HYPB/SETD2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HYPB/SETD2 stained by SETD2 Polyclonal Antibody (Product # PA5-34934) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETD2 Polyclonal Antibody detects SETD2 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. SETD2 Polyclonal Antibody (Product # PA5-34934) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 DMAPT induced histone H3K36 methyltransferases NSD1 and SETD2. ( a ) The effect of DMAPT on NSD1 expression. Cells were treated with 10 mu M DMAPT for 12 h, and qRT-PCR was performed to measure NSD1 mRNA. Average+-S.E.M. from biological replicates is shown. Asterisk in this and subsequent figures denotes statistically significant differences with a P -value of
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Immunohistochemical pattern in clear cell renal cell carcinomas displaying nuclear and/or cytoplasmic and/or membranous staining. a Snail, b Twist, c ZEB1, d beta-catenin, e E-cadherin, f Vimentin, g PTEN, h p110alpha, i p-Akt, j SETD2
