12231-1-AP

antibody from Proteintech Group

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Provider product page for 12231-1-AP

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Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [46]
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Validations
Western blot [1]

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Product number: 12231-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12231-1-AP, RRID:AB_2154081
Product name: NT5E/CD73 antibody
Antibody type: Polyclonal
Description: KD/KO validated NT5E/CD73 antibody (Cat. #12231-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NT5E protein structure - 12231-1-AP shown in red.

Submitted references Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion.

Lian W, Jiang D, Lin W, Jiang M, Zhang Y, Wang H, Zhao L
International journal of biological sciences 2024;20(1):137-151

Immune cell expression patterns of CD39/CD73 ectonucleotidases in rodent models of cardiac arrest and resuscitation.

Aoki T, Wong V, Yin T, Nakamura E, Endo Y, Hayashida K, Robson SC, Nandurkar H, Diamond B, Kim SJ, Murao A, Wang P, Becker LB, Shinozaki K
Frontiers in immunology 2024;15:1362858

Tumor cell senescence-induced macrophage CD73 expression is a critical metabolic immune checkpoint in the aging tumor microenvironment.

Deng Y, Chen Q, Yang X, Sun Y, Zhang B, Wei W, Deng S, Meng J, Hu Y, Wang Y, Zhang Z, Wen L, Huang F, Wan C, Yang K
Theranostics 2024;14(3):1224-1240

In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals.

Chung YH, Ho YP, Farn SS, Tsai WC, Li ZX, Lin TY, Weng CC
Biomedical journal 2024 Oct;47(5):100721

Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway.

Fu N, Zhang Z, Quan J
Biochemical and biophysical research communications 2024 May 14;708:149814

Mesenchymal stem cells-derived extracellular vesicle-incorporated H19 attenuates cardiac remodeling in rats with heart failure.

Jiao W, Hao J, Liu JM, Gao WN, Zhao JJ, Li YJ
The Kaohsiung journal of medical sciences 2024 Jan;40(1):46-62

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages.

Zhou L, Cai E, Liu H, Cheng H, Ye X, Zhu H, Chang X
Biochimica et biophysica acta. Molecular basis of disease 2024 Jan;1870(1):166895

Characterization of immunogenic cell death regulators predicts survival and immunotherapy response in lung adenocarcinoma.

Zhou D, Cui Y, Zhu M, Lin Y, Guo J, Li Y, Zhang J, Wu Z, Guo J, Chen Y, Liang W, Lin W, Lei K, Zhao T, You Q
Life sciences 2024 Feb 1;338:122396

Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler.

Hu P, Zhang G, Ba H, Ren J, Li J, Wang Z, Li C
Cellular & molecular biology letters 2024 Apr 20;29(1):56

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence.

Liu Z, Wu B, Liu X, Wu X, Du J, Xia G, Cai J, Zhu H, Sheng X, Zhang M, Xu J, Xu T, Lv X
International journal of biological sciences 2023;19(3):950-966

Dipyridamole activates adenosine A2B receptor and AMPK/cAMP signaling and promotes myogenic differentiation of myoblastic C2C12 cells.

Marco-Bonilla M, Herencia R, Fresnadillo M, Huete-Toral F, Carracedo G, Largo R, Herrero-Beaumont G, Mediero A
Frontiers in pharmacology 2023;14:1247664

A novel immunogenic cell death-related subtype classification and risk signature for predicting prognosis and immunotherapy efficacy in gastric cancer.

Dong B, Wu Y, Zhang J, Gu Y, Xie R, He X, Pang X, Wang X, Cui Y
Frontiers in immunology 2023;14:1162876

CD73 mitigates hepatic damage in alcoholic steatohepatitis by regulating PI3K/AKT-mediated hepatocyte pyroptosis.

Zhu H, Zhang M, Ye Y, Liu Z, Wang J, Wu X, Lv X
Biochemical pharmacology 2023 Sep;215:115753

CD73 inhibits titanium particle-associated aseptic loosening by alternating activation of macrophages.

Sun Z, Kang J, Yang S, Zhang Y, Huang N, Zhang X, Du G, Jiang J, Ning B
International immunopharmacology 2023 Sep;122:110561

Blocking ATP-P1Rs axis attenuate alcohol-related liver fibrosis.

Liu XQ, Wang JJ, Wu X, Liu ZN, Wu BM, Lv XW
Life sciences 2023 Sep 1;328:121896

Tumor Microenvironment Responsive CD8(+) T Cells and Myeloid-Derived Suppressor Cells to Trigger CD73 Inhibitor AB680-Based Synergistic Therapy for Pancreatic Cancer.

Chen Q, Yin H, He J, Xie Y, Wang W, Xu H, Zhang L, Shi C, Yu J, Wu W, Liu L, Pu N, Lou W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Nov;10(33):e2302498

Design, Synthesis and Biological Evaluation of Novel MDH Inhibitors Targeting Tumor Microenvironment.

Godesi S, Han JR, Kim JK, Kwak DI, Lee J, Nada H, Kim M, Yang HA, Im JY, Ban HS, Lee CH, Choi Y, Won M, Lee K
Pharmaceuticals (Basel, Switzerland) 2023 May 2;16(5)

METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3.

Hu B, Zou X, Yu Y, Jiang Y, Xu H
Regenerative therapy 2023 Mar;22:148-159

Norepinephrine inhibits CD8(+) T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma.

Geng Q, Li L, Shen Z, Zheng Y, Wang L, Xue R, Xue W, Peng M, Zhao J
British journal of cancer 2023 Mar;128(7):1223-1235

Roxadustat (FG-4592) protects against ischaemia-induced acute kidney injury via improving CD73 and decreasing AIM2 inflammasome activation.

Yang H, Wu Y, Cheng M, Zhang M, Qiu X, Liu S, Zhang M
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2023 Mar 31;38(4):858-875

Human amniotic mesenchymal stem cell-conditioned medium inhibited LPS-induced cell injury and inflammation by modulating CD14/TLR4-mediated signaling pathway in monocytes.

Li M, Li T, Jin J, Xie C, Zhu J
Molecular immunology 2023 Jun;158:10-21

Exosomes derived from TGF-β1-pretreated mesenchymal stem cells alleviate biliary ischemia-reperfusion injury through Jagged1/Notch1/SOX9 pathway.

Yasen A, Feng J, Xie XM, Li K, Cai YH, Liao ZH, Liang RB, Dai TX, Wang GY
International immunopharmacology 2023 Jun;119:110253

Primary cilia support cartilage regeneration after injury.

Tao D, Zhang L, Ding Y, Tang N, Xu X, Li G, Niu P, Yue R, Wang X, Shen Y, Sun Y
International journal of oral science 2023 Jun 2;15(1):22

Mesenchymal stem cell-derived conditioned medium and Methysergide give rise to crosstalk inhibition of 5-HT2A and 5-HT7 receptors in neuroblastoma cells.

Salkin H, Satir-Basaran G, Korkmaz S, Burcin Gonen Z, Erdem Basaran K
Brain research 2023 Jun 1;1808:148354

P2X7 receptor-mediated depression-like reactions arising in the mouse medial prefrontal cortex.

Ren WJ, Zhao YF, Li J, Rubini P, Yuan ZQ, Tang Y, Illes P
Cerebral cortex (New York, N.Y. : 1991) 2023 Jul 5;33(14):8858-8875

Mesenchymal stem cells, as glioma exosomal immunosuppressive signal multipliers, enhance MDSCs immunosuppressive activity through the miR-21/SP1/DNMT1 positive feedback loop.

Qiu W, Guo Q, Guo X, Wang C, Li B, Qi Y, Wang S, Zhao R, Han X, Du H, Zhao S, Pan Z, Fan Y, Wang Q, Gao Z, Li G, Xue H
Journal of nanobiotechnology 2023 Jul 22;21(1):233

Effects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cells.

Salkın H, Basaran KE
Connective tissue research 2023 Jan;64(1):14-25

Stem-cell based soft tissue substitutes: Engineering of crosslinked polylysine-hyaluronic acid microspheres ladened with gingival mesenchymal stem cells for collagen tissue regeneration and angiogenesis.

Ni J, Li M, Li C, Zhong Z, Xi H, Wu Y
Journal of periodontology 2023 Dec;94(12):1436-1449

CD73 Attenuates Alcohol-Induced Liver Injury and Inflammation via Blocking TLR4/MyD88/NF-κB Signaling Pathway.

Liu ZN, Wu X, Fang Q, Li ZX, Xia GQ, Cai JN, Lv XW
Journal of inflammation research 2022;15:53-70

Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats.

Nomura M, George J, Hashizume C, Saito T, Ueda Y, Ishigaki Y, Tsuchishima M, Tsutsumi M
Molecular medicine (Cambridge, Mass.) 2022 Nov 29;28(1):143

Identification of CD73 as a Novel Biomarker Encompassing the Tumor Microenvironment, Prognosis, and Therapeutic Responses in Various Cancers.

Tang K, Zhang J, Cao H, Xiao G, Wang Z, Zhang X, Zhang N, Wu W, Zhang H, Wang Q, Xu H, Cheng Q
Cancers 2022 Nov 17;14(22)

Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.

Wölkart G, Stessel H, Fassett E, Teschl E, Friedl K, Trummer M, Schrammel A, Kollau A, Mayer B, Fassett J
European journal of pharmacology 2022 Jul 15;927:175050

A novel type of mesenchymal stem cells derived from bovine metanephric mesenchyme.

Wang Y, Wu Y, Liu Y, Zhang Y, Yang X, Gao Y, Guan W
Tissue & cell 2022 Dec;79:101970

CD73 aggravates alcohol-related liver fibrosis by promoting autophagy mediated activation of hepatic stellate cells through AMPK/AKT/mTOR signaling pathway.

Wu X, Liu XQ, Liu ZN, Xia GQ, Zhu H, Zhang MD, Wu BM, Lv XW
International immunopharmacology 2022 Dec;113(Pt A):109229

Inhibitory Effect of Bovine Adipose-Derived Mesenchymal Stem Cells on Lipopolysaccharide Induced Inflammation of Endometrial Epithelial Cells in Dairy Cows.

Lu W, Xu ZM, Liu Q, Yu NN, Yu JB, Li WL, Mao YY, Du Z, Si L, Yuan S, Jin J, Fu S, Sun D, Han YH
Frontiers in veterinary science 2021;8:726328

GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.

Guo S, Gu J, Ma J, Xu R, Wu Q, Meng L, Liu H, Li L, Xu Y
Theranostics 2021;11(17):8379-8395

Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma.

Dell'Anno I, Martin SA, Barbarino M, Melani A, Silvestri R, Bottaro M, Paolicchi E, Corrado A, Cipollini M, Melaiu O, Giordano A, Luzzi L, Gemignani F, Landi S
Investigational new drugs 2021 Jun;39(3):644-657

PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E.

Wang X, Guo Q, Wang H, Yuan X, Wang B, Lobie PE, Zhu T, Tan S, Wu Z
Molecular cancer research : MCR 2021 Jan;19(1):86-98

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Hayashi M, Watanabe-Asaka T, Nagashio S, Kaidoh M, Yokoyama Y, Maejima D, Kajihara R, Amari K, Arai N, Kawai Y, Ohhashi T
American journal of physiology. Gastrointestinal and liver physiology 2021 Jan 1;320(1):G54-G65

A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.

Qi C, Deng Y, Xu L, Yang C, Zhu Y, Wang G, Wang Z, Wang L
Theranostics 2020;10(2):741-756

Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.

Lai Q, Yuan GY, Wang H, Liu ZL, Kou JP, Yu BY, Li F
Acta pharmacologica Sinica 2020 Aug;41(8):1058-1072

Metabolomic profiling of metoprolol-induced cardioprotection in a murine model of acute myocardial ischemia.

Lai Q, Yuan G, Wang H, Liu Z, Kou J, Yu B, Li F
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Apr;124:109820

Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin.

Ise H, Matsunaga K, Shinohara M, Sakai Y
Stem cells international 2019;2019:4341286

Mesenchymal stem cells overexpressing heme oxygenase-1 ameliorate lipopolysaccharide-induced acute lung injury in rats.

Chen X, Wu S, Tang L, Ma L, Wang F, Feng H, Meng J, Han Z
Journal of cellular physiology 2019 May;234(5):7301-7319

Targeting of NT5E by miR-30b and miR-340 attenuates proliferation, invasion and migration of gallbladder carcinoma.

Wang N, Xiang X, Chen K, Liu P, Zhu A
Biochimie 2018 Mar;146:56-67

Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.

Zhao XX, An XL, Zhu XC, Jiang Y, Zhai YH, Zhang S, Cai NN, Tang B, Li ZY, Zhang XM
Journal of experimental zoology. Part B, Molecular and developmental evolution 2018 Dec;330(8):406-416

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NT5E/CD73 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NT5E/CD73. This antibody recognizes human, mouse, rat antigen. The NT5E/CD73 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 12231-1-AP.pdf

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