Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5795 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human 5'-Nucleotidase/CD73 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse CD73 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references NAD(+) augmentation restores mitophagy and limits accelerated aging in Werner syndrome.
Fang EF, Hou Y, Lautrup S, Jensen MB, Yang B, SenGupta T, Caponio D, Khezri R, Demarest TG, Aman Y, Figueroa D, Morevati M, Lee HJ, Kato H, Kassahun H, Lee JH, Filippelli D, Okur MN, Mangerich A, Croteau DL, Maezawa Y, Lyssiotis CA, Tao J, Yokote K, Rusten TE, Mattson MP, Jasper H, Nilsen H, Bohr VA
Nature communications 2019 Nov 21;10(1):5284
Nature communications 2019 Nov 21;10(1):5284
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human 5'-Nucleotidase/CD73 by Western Blot. Detection of Human 5'-Nucleotidase/CD73 by Western Blot.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human 5'-Nucleotidase/CD73 by Simple WesternTM. Detection of Human 5'-Nucleotidase/CD73 by Simple WesternTM.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- 5'-Nucleotidase/CD73 in Human Cervical Cancer Tissue. 5'-Nucleotidase/CD73 in Human Cervical Cancer Tissue.