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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB5795P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells.
Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors.
Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
Li SJ, Luo Y, Zhang LM, Yang W, Zhang GG
Molecular medicine reports 2017 Mar;15(3):1313-1318
Molecular medicine reports 2017 Mar;15(3):1313-1318
Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors.
Rafiq QA, Coopman K, Nienow AW, Hewitt CJ
Biotechnology journal 2016 Mar;11(4):473-86
Biotechnology journal 2016 Mar;11(4):473-86
Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
Brady K, Dickinson SC, Guillot PV, Polak J, Blom AW, Kafienah W, Hollander AP
Stem cells and development 2014 Mar 1;23(5):541-54
Stem cells and development 2014 Mar 1;23(5):541-54
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of 5'-Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'-Nucleotidase/CD73 PE-conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
