Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB5795 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal 5'-Nucleotidase/CD73 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease.
Muhammad F, Wang D, Montieth A, Lee S, Preble J, Foster CS, Larson TA, Ding K, Dvorak JD, Lee DJ
Scientific reports 2019 Nov 15;9(1):16941
Scientific reports 2019 Nov 15;9(1):16941
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of 5'-Nucleotidase/CD73 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were labeled with Mouse Anti-Human/Equine 5'-Nucleotidase/CD73 Mono-clonal Antibody (Catalog # MAB5795) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of 5'-Nucleotidase/CD73 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Equine 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB5795, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
