41-0200

Lin TK, Chen SD, Chuang YC, Lan MY, Chuang JH, Wang PW, Hsu TY, Wang FS, Tsai MH, Huang ST, Wang XW, Tsai PC, Lin HY, Liou CW
Oxidative medicine and cellular longevity 2019;2019:9537504

Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity.

Ferreira ADF, Cunha PDS, Carregal VM, da Silva PC, de Miranda MC, Kunrath-Lima M, de Melo MIA, Faraco CCF, Barbosa JL, Frezard F, Resende V, Rodrigues MA, de Goes AM, Gomes DA
Stem cells international 2017;2017:9841035

Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells.

Madrigal A, Tan L, Zhao Y
Biological research 2017 Dec 26;50(1):43

Characterization of Tunneling Nanotubes in Wharton's jelly Mesenchymal Stem Cells. An Intercellular Exchange of Components between Neighboring Cells.

Sanchez V, Villalba N, Fiore L, Luzzani C, Miriuka S, Boveris A, Gelpi RJ, Brusco A, Poderoso JJ
Stem cell reviews and reports 2017 Aug;13(4):491-498

Blood-based markers of efficacy and resistance to cetuximab treatment in metastatic colorectal cancer: results from CALGB 80203 (Alliance).

Hatch AJ, Sibley AB, Starr MD, Brady JC, Jiang C, Jia J, Bowers DL, Pang H, Owzar K, Niedzwiecki D, Innocenti F, Venook AP, Hurwitz HI, Nixon AB, Alliance for Clinical Trials in Oncology
Cancer medicine 2016 Sep;5(9):2249-60

Dengue virus type 2 modulates endothelial barrier function through CD73.

Patkar C, Giaya K, Libraty DH
The American journal of tropical medicine and hygiene 2013 Jan;88(1):89-94

Mesenchymal markers on human adipose stem/progenitor cells.

Zimmerlin L, Donnenberg VS, Rubin JP, Donnenberg AD
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2013 Jan;83(1):134-40

Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing.

Haasters F, Prall WC, Anz D, Bourquin C, Pautke C, Endres S, Mutschler W, Docheva D, Schieker M
Journal of anatomy 2009 May;214(5):759-67

Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).

Thomson LF, Ruedi JM, Glass A, Moldenhauer G, Moller P, Low MG, Klemens MR, Massaia M, Lucas AH
Tissue antigens 1990 Jan;35(1):9-19

Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).

Thomson LF, Ruedi JM, Glass A, Moldenhauer G, Moller P, Low MG, Klemens MR, Massaia M, Lucas AH
Tissue antigens 1990 Jan;35(1):9-19

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CD73 Monoclonal Antibody (7G2) (Product # 41-0200) and a 63 kDa band corresponding to CD73 was observed across the cell lines tested except THP-1 and HeLa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), THP-1 (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4), SK-MEL-31 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody at a concentration of 1 µg/mL and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD73 Monoclonal Antibody (7G2) (Product # 41-0200) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human peripheral blood lymphocytes stained with Mouse anti-CD73 monoclonal antibody (Product # 41-0200).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL