PAB29314

antibody from Abnova Corporation

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29314

Provider

Abnova Corporation

Product name

NT5E polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human NT5E.

Antigen sequence

EFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQ
ISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPG
TNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGF
EMDKLIAQK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate, Lane 2: NBT-II cell lysate with NT5E polyclonal antibody (Cat# PAB29314) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence staining of U-2 OS cells with NT5E polyclonal antibody (Cat# PAB29314) under 1-4 ug/mL working concentration.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum tissue with NT5E polyclonal antibody (Cat# PAB29314) at 1:1000 - 1:2500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)