Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3845 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3845, RRID:AB_1578207
- Product name
- NT5E polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NT5E.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts.
Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia.
Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.
Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S
Journal of dental research 2003 Nov;82(11):888-92
Journal of dental research 2003 Nov;82(11):888-92
Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia.
Rosi F, Carlucci F, Marinello E, Tabucchi A
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2002 Mar;56(2):100-4
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2002 Mar;56(2):100-4
Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form.
Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y
European journal of biochemistry / FEBS 1990 Aug 17;191(3):563-9
European journal of biochemistry / FEBS 1990 Aug 17;191(3):563-9
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NT5E polyclonal antibody (Cat # PAB3845) in Y-79 cell line lysate (35 ug/lane). NT5E (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with NT5E polyclonal antibody (Cat # PAB3845) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
