MAB57951-100

antibody from R&D Systems

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB57951-100

Provider

R&D Systems

Product name

Human/Mouse/Rat 5'-Nucleotidase/CD73 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human 5'-Nucleotidase/CD73 in direct ELISAs. Detects human, mouse, and rat 5'-Nucleotidase/CD73 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P21589


Isotype

IgG

Antibody clone number

606117

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat 5'-Nucleotidase/CD73 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Huh-7 human hepatoma cell line, U266 human myeloma cell line, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 5'-Nucleotidase/CD73 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human 5'-Nucleotidase/CD73 by Simple Western^TM. Simple Western lane view shows lysates of U-251 MG human glioblastoma cell line and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for 5'-Nucleotidase/CD73 at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

5'-Nucleotidase/CD73 in Human Cervical Cancer Tissue. 5'-Nucleotidase/CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human/Mouse/Rat 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB57951) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.