Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10-4106 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CD73 Antibody
- Antibody type
- Monoclonal
- Description
- CD73, also known as ecto-5'-nucleotidase, is a glycosyl-phosphatidylinositol linked plasma membrane glycoprotein that is expressed on multiple cell types and in different tissues. It is a key enzyme in the regulation of purinergic signaling and inflammatory reactions. It hydrolyzes extracellular AMP into adenosine, which dampens immune cell activation, and reduces leukocyte trafficking CD73 potently suppresses antitumor T cell responses through its ability to generate adenosine. It has also emerged as an important regulator of tissue homeostasis and pathophysiologic responses related to immunity, inflammation, pain, ischemia, tissue fibrosis and cancer.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant CD73 p
rotein (amino acids 145-346) was us
ed as the immunogen for this antibo
dy.- Isotype
- IgG
- Antibody clone number
- ABM40E2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
No comments: Submit comment
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CD73. Anti- CD73 antibody (Clone: ABM40E2) was tested at 2 µg/ml on human liver lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD73 in renal cell carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD73 in endomatrium carcinoma using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD73 in human Liver using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD73 in human Spleen using CD73 antibody (Clone: ABM40E2) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell Surface flow analysis of hCD73 in PBMC (Lymphocytes) using 0.2µg/10^6 cells of CD73 clone ( ABM40E2). Green represents isotype control; red represents anti-hCD73 antibody. Goat anti-mouse PE conjugated secondary antibody (ABEOMICS) was used.
- Protocol
- Protocol