LS-C818678

antibody from LSBio

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C818678

Provider

LSBio

Product name

NT5E / eNT / CD73 Antibody (Preservative Free) LS-C818678

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Rat

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ratNT5E was immunoprecipitated using: Lane A: 0.5 mg A375 Whole Cell Lysate. 0.5 uL anti-ratNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratNT5E rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 72 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ratNT5E was immunoprecipitated using: Lane A: 0.5 mg A375 Whole Cell Lysate. 0.5 uL anti-ratNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratNT5E rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 72 kDa.