LS-C818678

antibody from LSBio

Targeting: NT5E CALJA, CD73, eN, eNT, NT5

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C818678

Provider

LSBio

Product name

NT5E / eNT / CD73 Antibody (Preservative Free) LS-C818678

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Rat

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-NT5E rabbit polyclonal antibody at 1:500 dilution. Lane A: Rat spleen tissue lysate. Lane B: Rat liver tissue lysate. Lysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 70 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ratNT5E was immunoprecipitated using: Lane A: 0.5 mg A375 Whole Cell Lysate. 0.5 uL anti-ratNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratNT5E rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 72 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ratNT5E was immunoprecipitated using: Lane A: 0.5 mg A375 Whole Cell Lysate. 0.5 uL anti-ratNT5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ratNT5E rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 72 kDa.