Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A02120-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-CD73/Nt5e Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for CD73/Nt5e detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides.
Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy.
Manna C, Das K, Mandal D, Banerjee D, Mukherjee J, Ganguly I, Naskar S, Bag S
Veterinary research communications 2023 Dec;47(4):2229-2233
Veterinary research communications 2023 Dec;47(4):2229-2233
Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy.
Liu P, Feng Y, Dong C, Yang D, Li B, Chen X, Zhang Z, Wang Y, Zhou Y, Zhao L
PloS one 2014;9(5):e97123
PloS one 2014;9(5):e97123
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CD73/Nt5e using anti-CD73/Nt5e antibody (A02120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD73/Nt5e antigen affinity purified polyclonal antibody (Catalog # A02120-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD73/Nt5e at approximately 63KD. The expected band size for CD73/Nt5e is at 63KD.
- Additional image