PA5-18189

antibody from Invitrogen Antibodies

Targeting: FBXW2 FBW2, Fwd2, Md6

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18189

Provider

Invitrogen Antibodies

Product name

FBXW2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine and porcine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

E3 Ligase FBXW2 Is a New Therapeutic Target in Obesity and Atherosclerosis.

Wang C, Xu W, Chao Y, Liang M, Zhang F, Huang K
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2020 Oct;7(20):2001800

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 FBXW2 expression in macrophages is upregulated from obese mice and humans. Eight-week-old C57BL/6J mice were fed either chow diet (CD) or high fat diet (HFD) for 12 weeks. A) FBXW2 expression in epiWAT was determined by western blot assay ( n = 8). B) Cell extracts from isolated adipocytes and SVFs in epiWAT were subject to western blot for the expression of FBXW2 ( n = 5). C) The protein level of FBXW2 in F4/80 + macrophages sorted from SVFs by western blot ( n = 5). D) Representative H&E staining of epiWAT, and the immunofluorescence images for CD68 (red) and FBXW2 (green) expression in epiWAT. Nuclei staining by DAPI (blue). Scale bars, 20 um. E) CD14 + macrophages were isolated from visceral adipose tissue of lean ( n = 33) and obese ( n = 42) subjects. The mRNA level of FBXW2 was tested by real-time qPCR assay. F) Representative H&E staining of visceral adipose tissues from lean and obese individuals, and the expressions of CD68 (red) and FBXW2 (green) were detected by immunofluorescence staining. Nuclei staining by DAPI (blue). Scale bars, 20 um. The data are shown as the mean +- SEM. * p < 0.05 by Student's t test or ANOVA with the post-hoc test.