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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX25425 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25425, RRID:AB_380526
- Product name
- EphB2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-EphB2 C-term Pab (GTX25425) in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of EphB2 Antibody (C-term) (GTX25425) with A375 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term) (GTX25425), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 Antibody (C-term) (GTX25425), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
