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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3020 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3020, RRID:AB_1574716
- Product name
- EPHB2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHB2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Oligomeric structure of the human EphB2 receptor SAM domain.
A variant transcript encoding an isoform of the human protein tyrosine kinase EPHB2 is generated by alternative splicing and alternative use of polyadenylation signals.
cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.
Thanos CD, Goodwill KE, Bowie JU
Science (New York, N.Y.) 1999 Feb 5;283(5403):833-6
Science (New York, N.Y.) 1999 Feb 5;283(5403):833-6
A variant transcript encoding an isoform of the human protein tyrosine kinase EPHB2 is generated by alternative splicing and alternative use of polyadenylation signals.
Tang XX, Pleasure DE, Brodeur GM, Ikegaki N
Oncogene 1998 Jul 30;17(4):521-6
Oncogene 1998 Jul 30;17(4):521-6
cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.
Fox GM, Holst PL, Chute HT, Lindberg RA, Janssen AM, Basu R, Welcher AA
Oncogene 1995 Mar 2;10(5):897-905
Oncogene 1995 Mar 2;10(5):897-905
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB2 polyclonal antibody (Cat # PAB3020) in NCI-H460 cell lysate. EPHB2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EPHB2 polyclonal antibody (Cat # PAB3020), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
