Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- STJ98032 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-EPHB2 antibody (17-200) [2D12C6] (STJ98032)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Ephrin Type-B Receptor 2 (17-200) is suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and ELISA research applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis using EphB2 monoclonal antibody against truncated EphB2 recombinant protein (1) and extracellular EphB2 (aa19-476) -hIgGFc transfected CHO-K1 cell lysate (2).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA