Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-43049 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADCY10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: VGHTVRHEYT VIGQKVNLAA RMMMYYPGIV TCDSVTYNGS NLPAYFFKEL Sequence homology: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Expression of Functional Cannabinoid Type-1 (CB(1)) Receptor in Mitochondria of White Adipocytes.
Pagano Zottola AC, Severi I, Cannich A, Ciofi P, Cota D, Marsicano G, Giordano A, Bellocchio L
Cells 2022 Aug 19;11(16)
Cells 2022 Aug 19;11(16)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Surface Plasmon Resonance of ADCY10 polyclonal antibody (Product # PA5-43049). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 µg/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.