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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-916 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-916, RRID:AB_2194939
- Product name
- Anti-SPANX (pan) (RABBIT) Antibody - 600-401-916
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
- Validation comment
- Western Blot
