24889-1-AP

antibody from Proteintech Group

Targeting: CYP7B1 SPG5A

Provider product page for 24889-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [37]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 24889-1-AP - Provider product page
Provider: Proteintech Group
Product name: CYP7B1 antibody
Antibody type: Polyclonal
Description: CYP7B1 antibody (Cat. #24889-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CYP7B1 protein structure - 24889-1-AP shown in red.

Submitted references High-fat diet perturbs hepatic lipid metabolism and impairs colonic autophagy to exacerbate experimental colitis.

Xu T, Shi J, Ji J, Ma C, Li J
3 Biotech 2026 May;16(5):171

AAV8-based gene replacement therapy for hereditary spastic paraplegia type 5.

Wiora L, Yuan Q, Hook S, Kraft M, Björkhem I, Ott M, Hauser S, Schöls L
Molecular therapy. Methods & clinical development 2025 Sep 11;33(3):101531

Celastrol ameliorates metabolic dysfunction associated steatohepatitis by regulating the CYP7B1-mediated alternative bile acid synthetic pathway.

Hu M, Yang J, Chen Y, Zhang Y, Liu CH, Feng Y, Ye B, Li D
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157172

3-O-Acetyloleanolic acid alleviates autoimmune liver injury by targeting FXR.

Wang Y, Li Z, Xiao W, Yongbo X, Li Y, Li Z, Chen D, Feng Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov 25;148:157273

A critical role for Phocaeicola vulgatus in negatively impacting metformin response in diabetes.

Chen M, Peng Y, Hu Y, Kang Z, Chen T, Zhang Y, Chen X, Li Q, Yuan Z, Wu Y, Xu H, Zhou G, Liu T, Zhou H, Yuan C, Huang W, Zhang W
Acta pharmaceutica Sinica. B 2025 May;15(5):2511-2528

Inhibition of SREBP-1c rescues hepatic CYP7B1 expression and bile acid synthesis in malnourished mice.

Wan X, Soni KG, Choi JM, Jung SY, Conner ME, Preidis GA
American journal of physiology. Gastrointestinal and liver physiology 2025 Jul 1;329(1):G232-G243

Hepatic FXR-FGF4 is required for bile acid homeostasis via an FGFR4-LRH-1 signal node under cholestatic stress.

Song L, Hou Y, Xu D, Dai X, Luo J, Liu Y, Huang Z, Yang M, Chen J, Hu Y, Chen C, Tang Y, Rao Z, Ma J, Zheng M, Shi K, Cai C, Lu M, Tang R, Ma X, Xie C, Luo Y, Li X, Huang Z
Cell metabolism 2025 Jan 7;37(1):104-120.e9

Hepatic Lamp2a deficiency promotes inflammation of murine autoimmune cholangitis via affecting bile acid metabolism.

Fan Q, Guo G, Hu Y, Lu Y, Su R, Yang J, Xia E, Ma S, Zhang M, Wang J, Li T, Han Y
iScience 2025 Feb 21;28(2):111804

Smilax china L. Extract Alleviates Metabolic-Associated Fatty Liver Disease by Regulating Gut Microbiota and Bile Acid Metabolism.

Cheng S, Li H, Sun Z, Xiong Y, Li J, Tian J, Shen Y, Shen L, Yang J, Yang Y, Liu D, Wei Q, Huang C, Ye X
Metabolites 2025 Dec 26;16(1)

Acalabrutinib alleviates metabolic dysfunction-associated steatotic liver disease by regulating bile acid metabolism.

Wang Y, Chen S, Ye B, Yang Z, Liu Y, Lou G, Zhou C, Zheng M
The international journal of biochemistry & cell biology 2025 Aug;185:106786

Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury.

Fan M, Xu Y, Wu B, Long J, Liu C, Liang Z, Zhang R, Liu Z, Wang C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(15):e2409107

Yellow tea polysaccharides protect against non-alcoholic fatty liver disease via regulation of gut microbiota and bile acid metabolism in mice.

Huang Y, Chen H, Chen J, Wu Q, Zhang W, Li D, Lu Y, Chen Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Oct;133:155919

The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation.

Jin M, Zou T, Huang H, Chen M, Zou H, Chen B, Lai C, Li H, Zhang P
Molecular nutrition & food research 2024 May;68(9):e2400147

Ganoderma lucidum polysaccharide ameliorates cholesterol gallstone formation by modulating cholesterol and bile acid metabolism in an FXR-dependent manner.

Huang D, Shen S, Zhuang Q, Ye X, Qian Y, Dong Z, Wan X
Chinese medicine 2024 Jan 24;19(1):16

ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism.

Luo J, Lu Z, Zhong Z, Pi M, Xiong Y, Li L, Chen T, Chen Y, Wang CY, Liu Z, Ye Q
Free radical biology & medicine 2024 Feb 20;212:34-48

Hepatic Gα13 ablation shifts region-specific colonic inflammatory status by modulating the bile acid synthetic pathway in mice.

Kwon SJ, Kim YS, Tak J, Lee SG, Lee EB, Kim SG
Scientific reports 2024 Aug 23;14(1):19580

Proteome characterization of liver-kidney comorbidity after microbial sepsis.

Gui Y, Yu Y, Wang W, Wang Y, Lu H, Mozdzierz S, Eskander K, Lin YH, Li H, Tian XJ, Liu S, Zhou D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Apr 15;38(7):e23597

High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia.

Tong H, Wang T, Chen R, Jin P, Jiang Y, Zhang X, Qi H
Experimental cell research 2024 Apr 1;437(1):113979

Loss of PPARα function promotes epigenetic dysregulation of lipid homeostasis driving ferroptosis and pyroptosis lipotoxicity in metabolic dysfunction associated Steatotic liver disease (MASLD).

Theys C, Vanderhaeghen T, Van Dijck E, Peleman C, Scheepers A, Ibrahim J, Mateiu L, Timmermans S, Vanden Berghe T, Francque SM, Van Hul W, Libert C, Vanden Berghe W
Frontiers in molecular medicine 2023;3:1283170

Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis.

Kuang J, Wang J, Li Y, Li M, Zhao M, Ge K, Zheng D, Cheung KCP, Liao B, Wang S, Chen T, Zhang Y, Wang C, Ji G, Chen P, Zhou H, Xie C, Zhao A, Jia W, Zheng X, Jia W
Cell metabolism 2023 Oct 3;35(10):1752-1766.e8

Theabrownin from Wuniuzao Dark Tea Regulates Hepatic Lipid Metabolism and Gut Microbiota in Mice Fed High-Fat Diet.

Xu Q, Ye J, Gong M, Zhang Y, Yuan Y, Zhao J
Nutrients 2023 Nov 24;15(23)

Stigmasterol Protects Against Steatohepatitis Induced by High-Fat and High-Cholesterol Diet in Mice by Enhancing the Alternative Bile Acid Synthesis Pathway.

Xin Y, Li X, Zhu X, Lin X, Luo M, Xiao Y, Ruan Y, Guo H
The Journal of nutrition 2023 Jul;153(7):1903-1914

Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen.

Xie J, Li H, Zhang X, Yang T, Yue M, Zhang Y, Chen S, Cui N, Yuan C, Li J, Zhu SJ, Liu W
Nature microbiology 2023 Jan;8(1):91-106

Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation.

Hu J, Hou Q, Zheng W, Yang T, Yan X
Journal of Zhejiang University. Science. B 2023 Aug 15;24(8):734-748

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Zhuang Q, Cheng J, Xia J, Ning M, Wu S, Shen S, Shi Y, Huang D, Dong Z, Wan X
Frontiers in medicine 2022;9:818144

FXR Signaling-Mediated Bile Acid Metabolism Is Critical for Alleviation of Cholesterol Gallstones by Lactobacillus Strains.

Ye X, Huang D, Dong Z, Wang X, Ning M, Xia J, Shen S, Wu S, Shi Y, Wang J, Wan X
Microbiology spectrum 2022 Oct 26;10(5):e0051822

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Fu Y, Feng H, Ding X, Meng QH, Zhang SR, Li J, Chao Y, Ji TT, Bi YH, Zhang WW, Chen Q, Zhang YH, Feng YL, Bian HM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jul;101:154120

Astragalus Polysaccharides Ameliorate Diet-Induced Gallstone Formation by Modulating Synthesis of Bile Acids and the Gut Microbiota.

Zhuang Q, Ye X, Shen S, Cheng J, Shi Y, Wu S, Xia J, Ning M, Dong Z, Wan X
Frontiers in pharmacology 2021;12:701003

Dysregulated oxalate metabolism is a driver and therapeutic target in atherosclerosis.

Liu Y, Zhao Y, Shukha Y, Lu H, Wang L, Liu Z, Liu C, Zhao Y, Wang H, Zhao G, Liang W, Fan Y, Chang L, Yurdagul A Jr, Pattillo CB, Orr AW, Aviram M, Wen B, Garcia-Barrio MT, Zhang J, Liu W, Sun D, Hayek T, Chen YE, Rom O
Cell reports 2021 Jul 27;36(4):109420

Gut microbiota mediates the effects of curcumin on enhancing Ucp1-dependent thermogenesis and improving high-fat diet-induced obesity.

Han Z, Yao L, Zhong Y, Xiao Y, Gao J, Zheng Z, Fan S, Zhang Z, Gong S, Chang S, Cui X, Cai J
Food & function 2021 Jul 21;12(14):6558-6575

Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.

Li W, Sun Z, Wu M, Deng Z, Zheng M, Kuang Z, Liu Y, He G
The Journal of nutritional biochemistry 2021 Dec;98:108868

Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.

Kakiyama G, Marques D, Martin R, Takei H, Rodriguez-Agudo D, LaSalle SA, Hashiguchi T, Liu X, Green R, Erickson S, Gil G, Fuchs M, Suzuki M, Murai T, Nittono H, Hylemon PB, Zhou H, Pandak WM
Journal of lipid research 2020 Dec;61(12):1629-1644

Downregulation of GPR183 on infection restricts the early infection and intracellular replication of mycobacterium tuberculosis in macrophage.

Tang J, Shi Y, Zhan L, Qin C
Microbial pathogenesis 2020 Aug;145:104234

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Chen Y, Zhou J, Xu S, Liu M, Wang M, Ma Y, Zhao M, Wang Z, Guo Y, Zhao L
Biochemical pharmacology 2019 Dec;170:113669

Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.

Zhong D, Xie Z, Huang B, Zhu S, Wang G, Zhou H, Lin S, Lin Z, Yang B
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;49(3):1163-1179

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Bai D, Li Q, Xiong Y, Wang C, Shen P, Bai L, Yuan L, Wu P
Toxicological sciences : an official journal of the Society of Toxicology 2018 Aug 1;164(2):501-511

Hydroxylation and sulfation of sex steroid hormones in inflammatory liver.

Lee SR, Lee SY, Kim SY, Ryu SY, Park BK, Hong EJ
Journal of biomedical research 2017 Sep 26;31(5):437-444

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP( CYP7B1 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CYP7B1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human CYP7B1. This antibody recognizes human, mouse antigen. The CYP7B1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 24889-1-AP.pdf

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