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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28121 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP7B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP7B1 Polyclonal Antibody (Product # PA5-28121). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: H1299. 10% SDS PAGE. CYP7B1 Polyclonal Antibody (Product # PA5-28121) diluted at 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CYP7B1 Polyclonal Antibody (Product # PA5-28121). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: H1299. 10% SDS PAGE. CYP7B1 Polyclonal Antibody (Product # PA5-28121) diluted at 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYP7B1 Polyclonal Antibody detects CYP7B1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP7B1 protein stained by CYP7B1 Polyclonal Antibody (Product # PA5-28121) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CYP7B1 Polyclonal Antibody detects CYP7B1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human liver carcinoma. CYP7B1 Polyclonal Antibody (Product # PA5-28121) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
