Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-9117-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD268 (BAFF Receptor) Monoclonal Antibody (8A7), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression. Applications Reported: The 8A7 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 8A7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 8A7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Type I Interferon Potentiates IgA Immunity to Respiratory Syncytial Virus Infection During Infancy.
IFN type I and II induce BAFF secretion from human decidual stromal cells.
BAFF promotes proliferation of human mesangial cells through interaction with BAFF-R.
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Hijano DR, Siefker DT, Shrestha B, Jaligama S, Vu LD, Tillman H, Finkelstein D, Saravia J, You D, Cormier SA
Scientific reports 2018 Jul 23;8(1):11034
Scientific reports 2018 Jul 23;8(1):11034
IFN type I and II induce BAFF secretion from human decidual stromal cells.
Lundell AC, Nordström I, Andersson K, Lundqvist C, Telemo E, Nava S, Kaipe H, Rudin A
Scientific reports 2017 Jan 6;7:39904
Scientific reports 2017 Jan 6;7:39904
BAFF promotes proliferation of human mesangial cells through interaction with BAFF-R.
Zheng N, Wang D, Ming H, Zhang H, Yu X
BMC nephrology 2015 May 15;16:72
BMC nephrology 2015 May 15;16:72
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Bornacelly A, Mercado D, Acevedo N, Caraballo L
BMC immunology 2014 Jun 7;15:22
BMC immunology 2014 Jun 7;15:22
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD268 (BAFF Receptor) FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye