Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-9117-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD268 (BAFF Receptor) Monoclonal Antibody (8A7), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression. Applications Reported: This 8A7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 8A7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8A7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Type I Interferon Potentiates IgA Immunity to Respiratory Syncytial Virus Infection During Infancy.
IFN type I and II induce BAFF secretion from human decidual stromal cells.
BAFF promotes proliferation of human mesangial cells through interaction with BAFF-R.
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
Hijano DR, Siefker DT, Shrestha B, Jaligama S, Vu LD, Tillman H, Finkelstein D, Saravia J, You D, Cormier SA
Scientific reports 2018 Jul 23;8(1):11034
Scientific reports 2018 Jul 23;8(1):11034
IFN type I and II induce BAFF secretion from human decidual stromal cells.
Lundell AC, Nordström I, Andersson K, Lundqvist C, Telemo E, Nava S, Kaipe H, Rudin A
Scientific reports 2017 Jan 6;7:39904
Scientific reports 2017 Jan 6;7:39904
BAFF promotes proliferation of human mesangial cells through interaction with BAFF-R.
Zheng N, Wang D, Ming H, Zhang H, Yu X
BMC nephrology 2015 May 15;16:72
BMC nephrology 2015 May 15;16:72
The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF).
Bornacelly A, Mercado D, Acevedo N, Caraballo L
BMC immunology 2014 Jun 7;15:22
BMC immunology 2014 Jun 7;15:22
A functional receptor for B-cell-activating factor is expressed on human acute lymphoblastic leukemias.
Parameswaran R, Müschen M, Kim YM, Groffen J, Heisterkamp N
Cancer research 2010 Jun 1;70(11):4346-56
Cancer research 2010 Jun 1;70(11):4346-56
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD268 (BAFF Receptor) APC (right). Cells in the lymphocyte gate were used for analysis.