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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- MA5-24334 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Axl (Tyr779) Monoclonal Antibody (713610)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 713610
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Axl (Y779) at approximately 150 kDa (as indicated) using 4 µg/mL of mouse Anti-human Phospho-Axl (Y779) Monoclonal Antibody (Product # MA5-24334). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.2 µg/mL of mouse Anti-human Phospho-Axl (Y779) Monoclonal Antibody (Product # MA5-24334) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Phospho-Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Axl (Tyr779) in A172 human glioblastoma cell line. Samples were incubated in Phospho-Axl (Tyr779) monoclonal antibody (Product # MA5-24334) using a dilution of 0.2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. Untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. A specific band was detected for Phospho-Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AXL in 0.2 mg/mL lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. Samples were incubated in AXL monoclonal antibody (Product # MA5-24334) using a dilution of 4 µg/mL. A specific band was detected for Phospho-Axl (Y779) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Phospho-Axl (Tyr779) in immersion fixed A172 human glioblastoma cells incubated with (upper panel) or without (lower panel) the phosphatase inhibitor pervanadate at 100 μM for 5 minutes. Samples were incubated in Phospho-Axl (Tyr779) monoclonal antibody (Product # MA5-24334) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A172 human glioblastoma cell line was unstimulated (open histogram) or treated with 100 µM pervanadate for 5 minutes, then stained with mouse Anti-human Phospho-Axl (Y779) Monoclonal Antibody (Product # MA5-24334, filled histo-gram), followed by Phycoerythrin-conjugated Anti-mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with Triton® X-100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Phospho-Axl (Tyr779) in A172 human glioblastoma cell line was unstimulated (open histogram) or treated with 100 µM pervanadate for 5 minutes. Samples were incubated in Phospho-Axl (Tyr779) monoclonal antibody (Product # MA5-24334) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody.To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with Triton® X-100.
