PA5-106118

antibody from Invitrogen Antibodies

Targeting: AXL ARK, JTK11, Tyro7, UFO

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-106118

Provider

Invitrogen Antibodies

Product name

Axl Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total AXL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice.

Al-Zaeed N, Budai Z, Szondy Z, Sarang Z
Cell death & disease 2021 Jun 12;12(6):611

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Axl in HUVEC cell lysate. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Axl polyclonal antibody (Product # PA5-106118) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Expression of TAM kinase family members and myogenic genes in the TA muscle of wild type and Mer mice. a mRNA expressions of myogenic marker genes Pax7, MyoD and MYHC1 in control and in regenerating wild type and Mer TA muscles determined by qRT-PCR at day 2, 4, or 10 post-CTX-induced injury. b mRNA expression levels of Mer, Axl and Tyro3 in control and in regenerating wild type and Mer TA muscles determined by qRT-PCR at day 2, 4, or 10 post-CTX-induced injury. c Protein levels of Mer, Axl, and Tyro3 in the TA muscle of wild-type and Mer mice determined by Western blot analysis. alpha Tauubulin was used as a loading control. Data are expressed as mean +- SEM ( n = 3). Asterisks indicate statistical significance (* P < 0.05, ** P < 0.01, ANOVA test).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 In vitro administration of the pan-TAM tyrosine kinase inhibitor BMS-777607 impairs myogenesis of C2C12 myoblast cells. a mRNA expression levels of Tyro3, Axl, and Mer during the differentiation of C2C12 myoblast cells determined by qRT-PCR. b Protein expression levels of Axl and myosin heavy chain 4 (MYHC4) in differentiating C2C12 myoblasts in the presence or absence of 1 muM BMS-777607 determined by Western blot analysis. alpha-Tubulin was used as a loading control. One representative blot of three is shown. c Alterations in the number of viable C2C12 cells grown in growth medium in the presence or absence of 1 muM BMS-777607 determined by PrestoBlue staining. d Percent of cells in G2/M phase grown in growth medium in the presence or absence of 1 muM BMS-777607 as indicated by anti-phospho-histone H3 (Ser10) and DAPI co-staining (at least 20 HPF were analyzed). Arrows point to the anti-phospho-histone H3 positive nuclei. e Percent of PI-positive cells grown in growth medium in the presence or absence of 1 muM BMS-777607. f Alterations in the number of viable C2C12 cells grown in differentiation medium in the presence or absence of 1 muM BMS-777607 determined by PrestoBlue staining. BMS-777607 was added when the medium was changed to differentiation medium. g Representative light microscopic images of C2C12 myoblasts differentiated for 6 days in the absence or presence of 1 muM BMS-777607 after staining dead cells with propidium iodide and quantification of propidium