PA5-48204

antibody from Invitrogen Antibodies

Targeting: DHCR7 SLOS

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-48204

Provider

Invitrogen Antibodies

Product name

DHCR7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.

Findakly S, Daggubati V, Garcia G, LaStella SA, Choudhury A, Tran C, Li A, Tong P, Garcia JQ, Puri N, Reiter JF, Xu L, Raleigh DR
The Journal of cell biology 2021 Jan 4;220(1)

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Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression.

Kohlhaas J, Jäger MA, Lust L, De La Torre C, Hecker M, Korff T
Experimental cell research 2021 Feb 15;399(2):112446

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of DHCR7 in MCF-7 cells. Samples probed with DHCR7 Antibody (C-term) (Product # PA5-48204) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclei (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of DHCR7 in HeLa cells. Cells were fixed, permeabilized and stained with DHCR7 polyclonal Antibody (Product # PA5-48204) at a 1:25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1:200 dilution (green). Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).