Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-48204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHCR7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway.
Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression.
Findakly S, Daggubati V, Garcia G, LaStella SA, Choudhury A, Tran C, Li A, Tong P, Garcia JQ, Puri N, Reiter JF, Xu L, Raleigh DR
The Journal of cell biology 2021 Jan 4;220(1)
The Journal of cell biology 2021 Jan 4;220(1)
Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression.
Kohlhaas J, Jäger MA, Lust L, De La Torre C, Hecker M, Korff T
Experimental cell research 2021 Feb 15;399(2):112446
Experimental cell research 2021 Feb 15;399(2):112446
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DHCR7 in mouse liver tissue lysates (35 µg/lane). Samples were probed with a DHCR7 Antibody (C-term) (Product # PA5-48204).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DHCR7 in (Lane 1) HepG2 whole cell lysate (20 µg), (Lane 2) Li-7 whole cell lysate (20 µg), (Lane 3) U-87 MG whole cell lysate (20 µg), (Lane 4) human testis lysate (20 µg), (Lane 5) mouse testis lysate (20 µg). Samples were probed with DHCR7 Antibody (C-term) (Product # PA5-48204) at 1:2000 dilution, followed by Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of DHCR7 in MCF-7 cells. Samples probed with DHCR7 Antibody (C-term) (Product # PA5-48204) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclei (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of DHCR7 in HeLa cells. Cells were fixed, permeabilized and stained with DHCR7 polyclonal Antibody (Product # PA5-48204) at a 1:25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1:200 dilution (green). Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DHCR7 in formalin fixed and paraffin embedded human testis tissue. Samples were probed with a DHCR7 Antibody (C-term) (Product # PA5-48204) followed by peroxidase conjugation of the secondary antibody and DAB staining.