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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- F54352 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DHCR7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 µl, 0.4 µl
- Storage
- Aliquot the DHCR7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) Li-7, 3) U-87 MG, 4) testis and 5) mouse testis lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse liver lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with DHCR7 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human MCF7 cells with DHCR7 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human testis tissue with DHCR7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
