Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARP56569_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP56569_P050, RRID:AB_10883105
- Product name
- Sema3f antibody - N-terminal region (ARP56569_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide corresponding to a region of Mouse
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Rabbit, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- WB Suggested Anti-Sema3f AntibodyTitration: 1.0µg/ml. Positive Control: Mouse Liver
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5µg Primary antibody dilution: 1:1000 Secondary antibody: Goat Anti-rabbit HRP Secondary antibody dilution:1:10000 - See more at: http://www.avivasysbio.com/anti-sema3f-antibody-n-terminal-region-arp56569-p050.html#sthash.vpQY9cNN.dpuf
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type : Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions : Gene Name : SEMA3FSubmitted by : Dr. Tracy Tran, Rutgers University