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Learn200-401-B48
antibody from Rockland Immunochemicals, Inc.
Targeting: CENPQ
C6orf139, CENP-Q, FLJ10545
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 200-401-B48 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-B48, RRID:AB_2076778
- Product name
- Anti-CENP-Q (RABBIT) Antibody - 200-401-B48
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Mammalian polo-like kinase 1-dependent regulation of the PBIP1-CENP-Q complex at kinetochores.
Kang YH, Park CH, Kim TS, Soung NK, Bang JK, Kim BY, Park JE, Lee KS
The Journal of biological chemistry 2011 Jun 3;286(22):19744-57
The Journal of biological chemistry 2011 Jun 3;286(22):19744-57
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy using Rockland's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD
- Sample type
- IF Microscopy
