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- References [3]
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- Product number
- MA5-17029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH2 Monoclonal Antibody (4G6A3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17029 targets ALDH2 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, and Rat samples. The MA5-17029 immunogen is purified recombinant fragment of human ALDH2 (amino acids: 317-517) expressed in E. Coli. MA5-17029 detects ALDH2 which has a predicted molecular weight of approximately 56.3kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4G6A3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A role for aldehyde dehydrogenase (ALDH) 2 in angiotensin II-mediated decrease in angiogenesis of coronary endothelial cells.
Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix.
AAV Gene Therapy for Alcoholism: Inhibition of Mitochondrial Aldehyde Dehydrogenase Enzyme Expression in Hepatoma Cells.
Roy B, Palaniyandi SS
Microvascular research 2021 May;135:104133
Microvascular research 2021 May;135:104133
Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix.
Ruan L, McNamara JT, Zhang X, Chang AC, Zhu J, Dong Y, Sun G, Peterson A, Na CH, Li R
Science advances 2020 Aug;6(32):eabc7288
Science advances 2020 Aug;6(32):eabc7288
AAV Gene Therapy for Alcoholism: Inhibition of Mitochondrial Aldehyde Dehydrogenase Enzyme Expression in Hepatoma Cells.
Sanchez AC, Li C, Andrews B, Asenjo JA, Samulski RJ
Human gene therapy 2017 Sep;28(9):717-725
Human gene therapy 2017 Sep;28(9):717-725
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALDH2 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with an ALDH2 mouse monoclonal antibody (Product # MA5-17029) at a concentration of 1.25 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Mitochondria (red) was stained with MitoTracker® Red CMXRos (Product # M7512). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALDH2 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with an ALDH2 mouse monoclonal antibody (Product # MA5-17029) at a concentration of 2.5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). F-actin (red) was stained by Dylight 554 Phalloidin (Product #21834) and nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using ALDH2 monoclonal antibody (Product # MA5-17029) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using ALDH2 monoclonal antibody (Product # MA5-17029) (green). Blue: fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ALDH2 monoclonal antibody (Product # MA5-17029) (green) and negative control (purple).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ALDH2 monoclonal antibody (Product # MA5-17029) (green) and negative control (red).
