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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX101429 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101429, RRID:AB_1949628
- Product name
- ALDH2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Proteomics of muscle chronological ageing in post-menopausal women.
β-catenin is essential for ethanol metabolism and protection against alcohol-mediated liver steatosis in mice.
Gueugneau M, Coudy-Gandilhon C, Gourbeyre O, Chambon C, Combaret L, Polge C, Taillandier D, Attaix D, Friguet B, Maier AB, Butler-Browne G, Béchet D
BMC genomics 2014 Dec 23;15:1165
BMC genomics 2014 Dec 23;15:1165
β-catenin is essential for ethanol metabolism and protection against alcohol-mediated liver steatosis in mice.
Liu S, Yeh TH, Singh VP, Shiva S, Krauland L, Li H, Zhang P, Kharbanda K, Ritov V, Monga SP, Scott DK, Eagon PK, Behari J
Hepatology (Baltimore, Md.) 2012 Mar;55(3):931-40
Hepatology (Baltimore, Md.) 2012 Mar;55(3):931-40
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALDH2 antibody detects ALDH2 protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g C8D30 whole cell lysate/extract C. 30 ?g BCL-1 whole cell lysae/extract D. 30 ?g Raw264.7 whole cell lysate/extract7.5% SDS-PAGEALDH2 antibody (GTX101429) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:H1299 12% SDS PAGEGTX101429 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HepG2 whole cell and cytoplasm extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 ?g/mL and GTX88073 for detection at a concentration of 1.5 ?g/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALDH2 antibody detects ALDH2 protein at mitochondria by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ALDH2 protein stained by ALDH2 antibody (GTX101429) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH2 antibody (GTX101429) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ALDH2 antibody (GTX101429) dilution: 1:500.
