MA5-32914

antibody from Invitrogen Antibodies

Targeting: ALDH2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32914

Provider

Invitrogen Antibodies

Product name

ALDH2 Monoclonal Antibody (E4-D10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

E4-D10

Vial size

100 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ALDH2 in HepG2 cell lysates using a ALDH2 Monoclonal antibody (Product # MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ALDH2 Monoclonal Antibody (E4-D10) (Product # MA5-32914) and a 56 kDa band corresponding to acetaldehyde dehydrogenase 2; ALDH2 was observed across the panel tested except for PANC-1, MCF7 and mouse brain which are reported to be low expressors of ALDH2. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), Hep G2 (Lane 2), A549 (Lane 3), PANC-1 (Lane 4), MCF7 (Lane 5), Mouse Liver (Lane 6), Mouse Lung (Lane 7), Mouse Brain (Lane 8), Rat Liver (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ALDH2 in HepG2 cells using a ALDH2 Monoclonal antibody (Product # MA5-32914)..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ALDH2 in PC-3M cells using a ALDH2 Monoclonal antibody (Product # MA5-32914)..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of acetaldehyde dehydrogenase 2; ALDH2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ALDH2 Monoclonal Antibody (E4-D10) (Product # MA5-32914) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:3000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondria localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of ALDH2 of paraffin-embedded Human lung cancer tissue using a ALDH2 Monoclonal antibody (Product #MA5-32914).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of ALDH2 in HepG2 cells using a ALDH2 Monoclonal Antibody (Product # MA5-32914) at a dilution of 1:100, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.