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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-27414 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: GL261, C8D30, BCL-1, Raw264.7, HepG2, HepG2 cytoplasm extract.
- Concentration
- 0.23 mg/mL
Submitted references In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
Aboulouard S, Wisztorski M, Duhamel M, Saudemont P, Cardon T, Narducci F, Lemaire AS, Kobeissy F, Leblanc E, Fournier I, Salzet M
Cell reports. Medicine 2021 Jun 15;2(6):100318
Cell reports. Medicine 2021 Jun 15;2(6):100318
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALDH2 using 30 µg of H1299 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ALDH2 polyclonal antibody (Product # PA5-27414) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH2 Polyclonal Antibody (Product # PA5-27414). HepG2 whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 Polyclonal Antibody (Product # PA5-27414) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ALDH2 Polyclonal Antibody (Product # PA5-27414) and a 56 kDa band corresponding to acetaldehyde dehydrogenase 2; ALDH2 was observed across the panel tested except for PANC-1, MCF7 and Mouse brain which are reported to be low expressors of ALDH2. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), Hep G2 (Lane 2), A549 (Lane 3), PANC-1 (Lane 4), MCF7 (Lane 5), Mouse Liver (Lane 6), Mouse Lung (Lane 7), Mouse Brain (Lane 8), Rat Liver (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg C8D30 whole cell lysate/extract. C. 30 µg BCL-1 whole cell lysae/extract. D. 30 µg Raw264.7 whole cell lysate/extract.7.5% SDS-PAGE. ALDH2 Polyclonal Antibody (Product # PA5-27414) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 Polyclonal Antibody (Product # PA5-27414) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of ALDH2 using ALDH2 Polyclonal Antibody (Product # PA5-27414) for capture at a concentration of 2.5 µg/mL and a second ALDH2 Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ALDH2 was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH2 Polyclonal Antibody (Product # PA5-27414) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ALDH2 Polyclonal Antibody (Product # PA5-27414) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2 (Product # PA5-27414) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH2 Polyclonal Antibody (Product # PA5-27414) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
