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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- ELISA [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-27414 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: GL261, C8D30, BCL-1, Raw264.7, HepG2, HepG2 cytoplasm extract. Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (82%), Pig (94%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.
Aboulouard S, Wisztorski M, Duhamel M, Saudemont P, Cardon T, Narducci F, Lemaire AS, Kobeissy F, Leblanc E, Fournier I, Salzet M
Cell reports. Medicine 2021 Jun 15;2(6):100318
Cell reports. Medicine 2021 Jun 15;2(6):100318
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of ALDH2 using ALDH2 Polyclonal Antibody (Product # PA5-27414) for capture at a concentration of 2.5 µg/mL and a second ALDH2 Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of ALDH2 using ALDH2 Polyclonal Antibody (Product # PA5-27414) for capture at a concentration of 2.5 µg/mL and a second ALDH2 Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ALDH2 was performed in A549 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH2 Polyclonal Antibody (Product # PA5-27414) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ALDH2 Polyclonal Antibody (Product # PA5-27414) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH2 Polyclonal Antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ALDH2 Polyclonal Antibody (Product # PA5-27414) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2 (Product # PA5-27414) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
