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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-117664 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.62 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of GLE1 in MCF-7 cells using GLE1 Polyclonal Antibody (Product # PA5-117664) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of GLE1 in MCF-7 cells using GLE1 Polyclonal Antibody (Product # PA5-117664) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating GLE1 in A549 whole cell lysate. Lane 1: Rabbit control IgG A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: GLE1 Polyclonal Antibody (Product # PA5-117664) (6 µg) + A549 whole cell lysate (500 µg). Lane 3: A549 whole cell lysate (20 µg).
