PA5-21816

antibody from Invitrogen Antibodies

Targeting: GLE1 GLE1L, hGLE1, LCCS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21816

Provider

Invitrogen Antibodies

Product name

GLE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using GLE1 Polyclonal Antibody (Product # PA5-21816). Sample (30 µg of whole cell lysate). A: HeLa. 7.5% SDS PAGE. GLE1 Polyclonal Antibody (Product # PA5-21816) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GLE1 in paraformaldehyde-fixed HeLa cells using a GLE1 polyclonal antibody (Product # PA5-21816) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GLE1 (Product # PA5-21816) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of GLE1 was performed in paraffin-embedded mouse brain tissue using GLE1 Polyclonal Antibody (Product # PA5-21816) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using GLE1 (Product # PA5-21816) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.