PA5-21712

antibody from Invitrogen Antibodies

Targeting: WNT11

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21712

Provider

Invitrogen Antibodies

Product name

WNT11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse heart. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (81%), Rabbit (97%), Chicken (85%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.39 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

WNT11, a new gene associated with early onset osteoporosis, is required for osteoblastogenesis.

Caetano da Silva C, Edouard T, Fradin M, Aubert-Mucca M, Ricquebourg M, Raman R, Salles JP, Charon V, Guggenbuhl P, Muller M, Cohen-Solal M, Collet C
Human molecular genetics 2022 May 19;31(10):1622-1634

---

16p11.2 transcription factor MAZ is a dosage-sensitive regulator of genitourinary development.

Haller M, Au J, O'Neill M, Lamb DJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 20;115(8):E1849-E1858

---

Role of Noncanonical Wnt Signaling Pathway in Human Aortic Valve Calcification.

Albanese I, Yu B, Al-Kindi H, Barratt B, Ott L, Al-Refai M, de Varennes B, Shum-Tim D, Cerruti M, Gourgas O, Rhéaume E, Tardif JC, Schwertani A
Arteriosclerosis, thrombosis, and vascular biology 2017 Mar;37(3):543-552

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of WNT11 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a WNT11 polyclonal antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of WNT11 in paraformaldehyde-fixed HeLa cells using a WNT11 polyclonal antibody (Product # PA5-21712) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WNT11 Polyclonal Antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11 antibody (Product # PA5-21712) at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WNT11 Polyclonal Antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 WNT11 heterozygous mutant cells with decreased WNT11 mRNA and protein levels as well as proliferation and mineralization. ( A ) Electropherogram showing the 32-bp deletion leading to a frameshift. fs * : frameshift. ( B ) RT-qPCR analysis of WNT11 mRNA expression in mutant versus control U2OS cells. Normalization was to GAPDH level as a housekeeping gene with ratio of one for control U2OS cells. Ctrl: wild-type U2OS cells, Mut: WNT11 mutant cells, FC: fold change. ( C ) Western blot analysis of WNT11 protein expression, confirming the heterozygosity. a-Tubulin was a control. ( D ) Alizarin red staining showed formation of mineralized nodules after osteogenic differentiation treatment with osteogenic media in control and WNT11 mutant cells. ( E ) Proliferation of control and WNT11 mutant cells ( n = 42). Data are mean +- SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001.