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LearnOAAB08112
antibody from Aviva Systems Biology
Targeting: BTRC
beta-TrCP1, betaTrCP, bTrCP, bTrCP1, FBXW1A, Fwd1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- OAAB08112 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- BTRC antibody - N - terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- BTRC Antibody (N-term) (Cat. #OAAB08112) western blot analysis in ZR-75-1 cell line lysates (35µg/lane).This demonstrates the BTRC antibody detected the BTRC protein (arrow).There is BioGPS gene expression data showing that BTRC is expressed in ZR75-1
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of BTRC Antibody (N-term)with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- BTRC Antibody (N-term) (Cat. #OAAB08112)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
