TA502465

antibody from OriGene

Targeting: BTRC beta-TrCP1, betaTrCP, bTrCP, bTrCP1, FBXW1A, Fwd1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502465

Provider

OriGene

Proper citation

OriGene Cat#TA502465, RRID:AB_2622370

Product name

BTRC (Beta TRCP) mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)

Antibody type

Monoclonal

Description

BTRC (Beta TRCP) mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)

Host

Mouse

Conjugate

Unconjugated

Epitope

BTRC

Isotype

IgG

Antibody clone number

OTI2H2

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTRC (RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTRC monoclonal antibody at 1:200 dilution.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line by using anti-BTRC monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BTRC mouse monoclonal antibody (TA502465) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTRC(RC207025).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC207025 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTRC antibody(TA502465), and then analyzed by flow cytometry.

Validation comment

FC