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LearnCF502465
antibody from Invitrogen Antibodies
Targeting: BTRC
beta-TrCP1, betaTrCP, bTrCP, bTrCP1, FBXW1A, Fwd1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- CF502465 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BTRC Monoclonal Antibody (OTI2H2), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2H2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTRC (RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 3 different cell lines by using anti-BTRC monoclonal antibody at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from a mouse cell line by using anti-BTRC monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-BTRC mouse monoclonal antibody (TA502465) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTRC(RC207025).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207025 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTRC antibody (TA502465), and then analyzed by flow cytometry.
