600-401-677S
antibody from Rockland Immunochemicals, Inc.
Targeting: AURKB
Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5
Antibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-677S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-677S, RRID:AB_11183440
- Product name
- Anti-Aurora B pT232 (RABBIT) Antibody - 600-401-677S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references METTL16-mediated N6-methyladenosine modification of Soga1 enables proper chromosome segregation and chromosomal stability in colorectal cancer.
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
An Aurora B-RPA signaling axis secures chromosome segregation fidelity.
PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size.
DENND2B activates Rab35 at the intercellular bridge, regulating cytokinetic abscission and tetraploidy.
Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation.
A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation.
VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitro.
Wip1 controls the translocation of the chromosomal passenger complex to the central spindle for faithful mitotic exit.
Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing.
Cell division requires RNA eviction from condensing chromosomes.
Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence.
BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.
Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
Chromosome Segregation Is Biased by Kinetochore Size.
Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway.
Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription.
Plk1 overexpression induces chromosomal instability and suppresses tumor development.
USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.
Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.
Studying Kinetochore Kinases.
IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells.
The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation.
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4.
TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation.
Negative feedback at kinetochores underlies a responsive spindle checkpoint signal.
Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres.
Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex.
Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint.
Li J, Yang F, Wang Z, Zheng S, Zhang S, Wang C, He B, Wang JB, Wang H
Cell proliferation 2024 May;57(5):e13590
Cell proliferation 2024 May;57(5):e13590
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
Song H, Kim EH, Hong J, Gwon D, Kim JW, Bae GU, Jang CY
Cell death and differentiation 2023 Sep;30(9):2151-2166
Cell death and differentiation 2023 Sep;30(9):2151-2166
An Aurora B-RPA signaling axis secures chromosome segregation fidelity.
Roshan P, Kuppa S, Mattice JR, Kaushik V, Chadda R, Pokhrel N, Tumala BR, Biswas A, Bothner B, Antony E, Origanti S
Nature communications 2023 May 25;14(1):3008
Nature communications 2023 May 25;14(1):3008
PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size.
Sobajima T, Kowalczyk KM, Skylakakis S, Hayward D, Fulcher LJ, Neary C, Batley C, Kurlekar S, Roberts E, Gruneberg U, Barr FA
The Journal of cell biology 2023 May 1;222(5)
The Journal of cell biology 2023 May 1;222(5)
DENND2B activates Rab35 at the intercellular bridge, regulating cytokinetic abscission and tetraploidy.
Kumar R, Francis V, Ioannou MS, Aguila A, Khan M, Banks E, Kulasekaran G, McPherson PS
Cell reports 2023 Jul 25;42(7):112795
Cell reports 2023 Jul 25;42(7):112795
Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation.
Jiang H, Kong N, Liu Z, West SC, Chan YW
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Apr;10(12):e2204388
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Apr;10(12):e2204388
A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents.
LaJoie D, Turkmen AM, Mackay DR, Jensen CC, Aksenova V, Niwa M, Dasso M, Ullman KS
Molecular biology of the cell 2022 Nov 1;33(13):ar117
Molecular biology of the cell 2022 Nov 1;33(13):ar117
SKAP interacts with Aurora B to guide end-on capture of spindle microtubules via phase separation.
Zhang M, Yang F, Wang W, Zohbi N, Wang X, Wang D, Zhuang X, Dou Z, Liu D, Song X, Green HN, Liu X, Yao X
Journal of molecular cell biology 2022 Jan 29;13(12):841-852
Journal of molecular cell biology 2022 Jan 29;13(12):841-852
VTT-006, an anti-mitotic compound, binds to the Ndc80 complex and suppresses cancer cell growth in vitro.
Laine LJ, Mäki-Jouppila JHE, Kutvonen E, Tiikkainen P, Nyholm TKM, Tien JF, Umbreit NT, Härmä V, Kallio L, Davis TN, Asbury CL, Poso A, Gorbsky GJ, Kallio MJ
Oncoscience 2021;8:134-153
Oncoscience 2021;8:134-153
Wip1 controls the translocation of the chromosomal passenger complex to the central spindle for faithful mitotic exit.
Zhang X, Park JE, Kim EH, Hong J, Hwang KT, Kim YA, Jang CY
Cellular and molecular life sciences : CMLS 2021 Mar;78(6):2821-2838
Cellular and molecular life sciences : CMLS 2021 Mar;78(6):2821-2838
Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing.
Strohacker LK, Mackay DR, Whitney MA, Couldwell GC, Sundquist WI, Ullman KS
eLife 2021 Aug 4;10
eLife 2021 Aug 4;10
Cell division requires RNA eviction from condensing chromosomes.
Sharp JA, Perea-Resa C, Wang W, Blower MD
The Journal of cell biology 2020 Nov 2;219(11)
The Journal of cell biology 2020 Nov 2;219(11)
Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence.
Barroso-Vilares M, Macedo JC, Reis M, Warren JD, Compton D, Logarinho E
EMBO reports 2020 May 6;21(5):e49248
EMBO reports 2020 May 6;21(5):e49248
BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.
Huang Y, Lin L, Liu X, Ye S, Yao PY, Wang W, Yang F, Gao X, Li J, Zhang Y, Zhang J, Yang Z, Liu X, Yang Z, Zang J, Teng M, Wang Z, Ruan K, Ding X, Li L, Cleveland DW, Zhang R, Yao X
Cell research 2019 Jul;29(7):562-578
Cell research 2019 Jul;29(7):562-578
Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability.
Achuthankutty D, Thakur RS, Haahr P, Hoffmann S, Drainas AP, Bizard AH, Weischenfeldt J, Hickson ID, Mailand N
The Journal of cell biology 2019 Dec 2;218(12):3943-3953
The Journal of cell biology 2019 Dec 2;218(12):3943-3953
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Liu S, Kwon M, Mannino M, Yang N, Renda F, Khodjakov A, Pellman D
Nature 2018 Sep;561(7724):551-555
Nature 2018 Sep;561(7724):551-555
Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
Weith M, Seiler J, van den Boom J, Kracht M, Hülsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Molecular cell 2018 Nov 15;72(4):766-777.e6
Molecular cell 2018 Nov 15;72(4):766-777.e6
Chromosome Segregation Is Biased by Kinetochore Size.
Drpic D, Almeida AC, Aguiar P, Renda F, Damas J, Lewin HA, Larkin DM, Khodjakov A, Maiato H
Current biology : CB 2018 May 7;28(9):1344-1356.e5
Current biology : CB 2018 May 7;28(9):1344-1356.e5
Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway.
Xie CM, Lin XT, Wu D, Tan Y, Cheng CHK, Zhang J
Oncotarget 2018 Mar 2;9(17):13783-13795
Oncotarget 2018 Mar 2;9(17):13783-13795
Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription.
Novais-Cruz M, Alba Abad M, van IJcken WF, Galjart N, Jeyaprakash AA, Maiato H, Ferrás C
eLife 2018 Aug 6;7
eLife 2018 Aug 6;7
Plk1 overexpression induces chromosomal instability and suppresses tumor development.
de Cárcer G, Venkateswaran SV, Salgueiro L, El Bakkali A, Somogyi K, Rowald K, Montañés P, Sanclemente M, Escobar B, de Martino A, McGranahan N, Malumbres M, Sotillo R
Nature communications 2018 Aug 1;9(1):3012
Nature communications 2018 Aug 1;9(1):3012
USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability.
Skowyra A, Allan LA, Saurin AT, Clarke PR
Cell reports 2018 Apr 17;23(3):852-865
Cell reports 2018 Apr 17;23(3):852-865
Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells.
Shrestha RL, Conti D, Tamura N, Braun D, Ramalingam RA, Cieslinski K, Ries J, Draviam VM
Nature communications 2017 Jul 28;8(1):150
Nature communications 2017 Jul 28;8(1):150
Studying Kinetochore Kinases.
Saurin AT, Kops GJ
Methods in molecular biology (Clifton, N.J.) 2016;1413:333-47
Methods in molecular biology (Clifton, N.J.) 2016;1413:333-47
IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells.
Lee S, Jeong AL, Park JS, Han S, Jang CY, Kim KI, Kim Y, Park JH, Lim JS, Lee MS, Yang Y
Cellular and molecular life sciences : CMLS 2016 Sep;73(17):3375-86
Cellular and molecular life sciences : CMLS 2016 Sep;73(17):3375-86
The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Redli PM, Gasic I, Meraldi P, Nigg EA, Santamaria A
The Journal of cell biology 2016 Oct 10;215(1):77-93
The Journal of cell biology 2016 Oct 10;215(1):77-93
Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation.
Mo F, Zhuang X, Liu X, Yao PY, Qin B, Su Z, Zang J, Wang Z, Zhang J, Dou Z, Tian C, Teng M, Niu L, Hill DL, Fang G, Ding X, Fu C, Yao X
Nature chemical biology 2016 Apr;12(4):226-32
Nature chemical biology 2016 Apr;12(4):226-32
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation.
Asghar A, Lajeunesse A, Dulla K, Combes G, Thebault P, Nigg EA, Elowe S
Nature communications 2015 Sep 24;6:8364
Nature communications 2015 Sep 24;6:8364
ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4.
Thoresen SB, Campsteijn C, Vietri M, Schink KO, Liestøl K, Andersen JS, Raiborg C, Stenmark H
Nature cell biology 2014 Jun;16(6):550-60
Nature cell biology 2014 Jun;16(6):550-60
TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation.
Ohishi T, Muramatsu Y, Yoshida H, Seimiya H
Molecular and cellular biology 2014 Jul;34(13):2464-78
Molecular and cellular biology 2014 Jul;34(13):2464-78
Negative feedback at kinetochores underlies a responsive spindle checkpoint signal.
Nijenhuis W, Vallardi G, Teixeira A, Kops GJ, Saurin AT
Nature cell biology 2014 Dec;16(12):1257-64
Nature cell biology 2014 Dec;16(12):1257-64
Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres.
Carretero M, Ruiz-Torres M, Rodríguez-Corsino M, Barthelemy I, Losada A
The EMBO journal 2013 Nov 13;32(22):2938-49
The EMBO journal 2013 Nov 13;32(22):2938-49
Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex.
Rivera T, Ghenoiu C, Rodríguez-Corsino M, Mochida S, Funabiki H, Losada A
The EMBO journal 2012 Mar 21;31(6):1467-79
The EMBO journal 2012 Mar 21;31(6):1467-79
Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint.
Mackay DR, Makise M, Ullman KS
The Journal of cell biology 2010 Nov 29;191(5):923-31
The Journal of cell biology 2010 Nov 29;191(5):923-31
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All lanes : Aurora B (phospho T232) antibody diluted 1:500. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen.
- Validation comment
- Western Blot