H00001636-M01

antibody from Abnova Corporation

Targeting: ACE ACE1, CD143, DCP1

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001636-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001636-M01, RRID:AB_518705

Product name

ACE monoclonal antibody (M01), clone 6A4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ACE.

Antigen sequence

RLATAMKLGFSRPWPEAMQLITGQPNMSASAMLSY
FKPLLDWLRTENELHGEKLGWPQYNWTPNSDDFYN
ETETKIFLQFYDQTGIWDHGAPHLLPPSQARGTRE
APVYM


Isotype

IgG

Antibody clone number

6A4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly.

Cargnello M, Tcherkezian J, Dorn JF, Huttlin EL, Maddox PS, Gygi SP, Roux PP
Molecular and cellular biology 2012 Nov;32(22):4572-84

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The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography.

Cougot N, Cavalier A, Thomas D, Gillet R
Journal of molecular biology 2012 Jun 29;420(1-2):17-28

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Drosophila genome-wide RNAi screen identifies multiple regulators of HIF-dependent transcription in hypoxia.

Dekanty A, Romero NM, Bertolin AP, Thomas MG, Leishman CC, Perez-Perri JI, Boccaccio GL, Wappner P
PLoS genetics 2010 Jun 24;6(6):e1000994

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ACE is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol